摘要： 研究了丁香油、间氨基苯甲酸乙酯甲磺酸盐（MS-222）和异丙酚对养殖大黄鱼的麻醉效果，以期为下一步活体运输麻醉剂的选择提供参考。首先在20（±0.5） ℃水温下，对3种麻醉剂的适宜质量浓度进行了探索，初步得出适宜的质量浓度分别为：丁香油10 mg/L、MS-222 40 mg/L、异丙酚0.2 mg/L。在此质量浓度下，进一步优化了大黄鱼的适宜麻醉水温。结果显示：丁香油在20 ℃、MS-22215在 ℃、异丙酚在25 ℃的麻醉效果最好。根据优化的质量浓度和水温，分别对3种麻醉剂麻醉后的大黄鱼进行模拟运输，发现随着模拟运输时间的延长，3种麻醉剂麻醉后的大黄鱼复苏率均逐渐降低，模拟运输24 h后丁香油和MS-222麻醉的复苏率可达90 %，而异丙酚麻醉的复苏率为70 %，说明丁香油和MS-222的麻醉效果较好。对丁香油和MS-222经济成本分析表明，丁香油的成本相对较低，更适合作为大黄鱼活体运输的麻醉剂。

关键词: 大黄鱼, 活鱼运输, 麻醉剂, 丁香油, MS-222, 异丙酚 ,  

Abstract: The anesthetic effects of clove oil, MS-222 and propofol on aquaculture of Larimichthys crocea were studied to provide a reference in the selection of anesthetics for live transportation. At the water temperature of 20 (±0.5) ℃, the appropriate mass concentrations of three anesthetics were firstly explored, and the appropriate mass concentrations were preliminarily obtained as follows: clove oil: 10 mg/L, MS-222: 40 mg/L, and propofol: 0.2 mg/L. Subsequently, the suitable water temperature for anesthesia of Larimichthys crocea was optimized, and the results showed that when clove oil was at 20 ℃, MS-222 at 15 ℃, and propofol at 25 ℃, the anesthetic effect was the best. According to the optimized mass concentration and water temperature, the simulated transportation of Larimichthys crocea anesthetized with three anesthetics was carried out. It was found that with the extension of simulated transportation time, the recovery rate of Larimichthys crocea anesthetized with the three anesthetics gradually decreased. After 24 h, the recovery rate of Larimichthys crocea anesthetized with clove oil and MS-222 could reach 90 %, but the recovery rate of Larimichthys crocea anesthetized with propofol was 70 %, indicating the anesthetic effects of clove oil and MS-222 were better. The economic cost analysis of clove oil and MS-222 shows that the cost of clove oil is relatively low, so it is more suitable to be used as an anesthetic for live transportation of Larimichthys crocea.    []

Key words:  Larimichthys crocea, live fish transportation, anesthetic, clove oil, MS-222, propofol