Accurately understanding of oxygen consumption characteristics and the key influencing factors of the fish is one of the important requirements for the design, construction, and efficient operation of an industrialized culture system. In the paper, the oxygen consumption rate of Takifugu rubripes with a body mass of (311 ± 11.22) g has been tested under different conditions by using a homemade testing device. In consideration of uncontrollable feeding conditions, all of the testing fish are fed by injection after anesthesia. There are one control group (completely unfed), two water temperature experimental groups (20 ℃ and 28 ℃), and four feeding rate experimental groups (0%, 0.3%, 0.6%, and 1.2% of body weight, respectively) were designed in the study. The results showed that under the unfed condition, the standard body mass oxygen consumption rates of Takifugu rubripes under two water temperatures were (70.89 ± 22.21) mg O2/ (kg•h) and (211.49 ± 37.67) mg O2/(kg•h), respectively. The effect of water temperature was significantly different (P < 0.01), but there was no obvious influence by circadian rhythm (P＞0.05). Under the feeding condition, the peak oxygen consumption rate was about 1.7 ~ 2 times that of the 0% experimental group at the same temperature.Tthe higher the feeding rate, the shorter it is required for the oxygen consumption rate to reach the peak, which is not obvious at 20℃. As the recovery time of oxygen consumption rate, it is about 2.5 -7.5 hours at 20 ℃ and 3 - 15 hours at 28 ℃. There is obvious that the recovery time increases significantly with higher water temperature and feeding rate. Therefore, the study shows that Takifugu rubripes will extend their oxygen consumption rate recovery time to meet their higher feeding energy consumption, not the peak oxygen consumption rate. The study systematically evaluates the effects of water temperature and feeding conditions on oxygen consumption of Takifugu rubripes, which can provide a reference for the management and operation of industrialized aquaculture systems and provide the theoretical basis for accurate control of dissolved oxygen in high-density aquaculture systems.