Abstract: Accurately understanding of oxygen consumption characteristics and the key influencing factors of the fish is one of the important requirements for the design, construction, and efficient operation of an industrialized culture system. In the paper, the oxygen consumption rate of Takifugu rubripes with a body mass of (311 ± 11.22) g has been tested under different conditions by using a homemade testing device. In consideration of uncontrollable feeding conditions, all of the testing fish are fed by injection after anesthesia. There are one control group (completely unfed), two water temperature experimental groups (20 ℃ and 28 ℃), and four feeding rate experimental groups (0%, 0.3%, 0.6%, and 1.2% of body weight, respectively) were designed in the study. The results showed that under the unfed condition, the standard body mass oxygen consumption rates of Takifugu rubripes under two water temperatures were (70.89 ± 22.21) mg O2/ (kg•h) and (211.49 ± 37.67) mg O2/(kg•h), respectively. The effect of water temperature was significantly different (P < 0.01), but there was no obvious influence by circadian rhythm (P＞0.05). Under the feeding condition, the peak oxygen consumption rate was about 1.7 ~ 2 times that of the 0% experimental group at the same temperature.Tthe higher the feeding rate, the shorter it is required for the oxygen consumption rate to reach the peak, which is not obvious at 20℃. As the recovery time of oxygen consumption rate, it is about 2.5 -7.5 hours at 20 ℃ and 3 - 15 hours at 28 ℃. There is obvious that the recovery time increases significantly with higher water temperature and feeding rate. Therefore, the study shows that Takifugu rubripes will extend their oxygen consumption rate recovery time to meet their higher feeding energy consumption, not the peak oxygen consumption rate. The study systematically evaluates the effects of water temperature and feeding conditions on oxygen consumption of Takifugu rubripes, which can provide a reference for the management and operation of industrialized aquaculture systems and provide the theoretical basis for accurate control of dissolved oxygen in high-density aquaculture systems.

Key words:  industrial aquaculture, takifugu rubripes, puffer, oxygen consumption, feeding

摘要： 系统全面地了解红鳍东方鲀耗氧特性，掌握其关键影响因子和变化规律是工厂化养殖系统设计构建和高效运行的重要基础之一。研究使用自制耗氧率测试装置，采用麻醉灌喂方法测试了体质量(311±11.22)g的红鳍东方鲀在不同条件下的耗氧率情况。共设计1个对照组（完全禁食）、2个水温试验组（分别为20℃和28℃）和4个摄食率试验组（分别为体质量的0%、0.3%、0.6%和1.2%），通过加热棒控制测试装置内的水温，通过传感器实时监测并记录溶解氧变化情况，并计算出红鳍东方鲀标准体质量耗氧率。结果显示：禁食状态下，红鳍东方鲀在水温20℃时的标准体质量耗氧率为（70.89±22.21）mg O2/(kg•h)，在28℃时为（211.49±37.67）mg O2/(kg•h)，水温影响差异显著（P＜0.01），但是没有表现出明显的昼夜节律；摄食状态下，红鳍东方鲀摄食率对于耗氧率峰值没有显著影响，是0%试验组的1.7~2倍。高温情况下摄食率越高，耗氧率到达峰值所需时间越短，低温情况下并不明显；从耗氧率回复时间来看，20℃时回复时间为2.5~7.5 h，28℃时为3~15 h，存在随着水温和摄食率的提高，回复时间明显增加的趋势。研究认为红鳍东方鲀主要通过延长耗氧率回复时间来满足其更高的摄食耗能。本研究系统阐述了水温和摄食条件对红鳍东方鲀耗氧的影响，为工厂化养殖系统管理运行及高密度养殖系统溶氧精准调控提供参考。

关键词: 工厂化养殖, 红鳍东方鲀, 河鲀, 耗氧, 摄食