Abstract: As a strong oxidant, ozone is often used as a bactericidal disinfectant for MBBR systems. In order to obtain the effect of ozone on the water quality of MBBR treatment and on the bactericidal rate of Vibrio parahaemolyticus, a 10 L reactor was used to determine the nitrification capacity of the system and the number of viable dead cells of the biofilm samples under a 10 L reactor at 60% filling rate to study the effects of different concentrations of ozone (0、1、2、4、6 mg/L) on the nitrification capacity of the system and the killing of Vibrio parahaemolyticus. The results showed that 2 mg/L ozone had no significant effect on cell mortality in biofilm sample (P = 0.49). The removal rates of TAN were 55.11% ± 5.32%, 57.89% ± 9.01%, 49.2% ± 5.99%, 33.07% ± 2.68%, 34.22% ± 8.89%, and the 4 mg/L ozone dose group was significantly lower than that of 0、1、2 mg/L ozone dose group (P = 0.042). At ozone doses above 2 mg/L, Vibrio parahaemolyticus was not detected on TCBS plates. This study showed that according to the mortality rate of biofilm cells, the treatment efficiency of ammonia nitrogen and the sterilization rate of Vibrio parahaemolyticus. The 2 mg/L ozone can effectively kill Vibrio parahaemolyticus and do not affect the nitration reaction efficiency of MBBR. 

Key words: MBBR, ozone, nitrify, Vibrio parahaemolyticus

摘要： 臭氧作为一种强氧化剂，常用作移动床生物膜反应器（Moving bed biofilm reactor，MBBR）系统的杀菌消毒剂。为了得到臭氧对MBBR处理水质的影响与对副溶血弧菌的杀菌效果，用10 L反应器，在60%填充率下，研究不同质量浓度臭氧（0、1、2、4、6 mg/L）对系统硝化能力与对副溶血弧菌（Vibrio parahaemolyticus）的杀灭效果。结果显示：与对照组相比，2 mg/L臭氧对滤料细胞死亡率影响不显著（P = 0.49）；总氨氮（TAN）去除率分别为55.11% ± 5.32%、57.89 %± 9.01%、49.2% ± 5.99%、33.07% ± 2.68%、34.22% ± 8.89%；当臭氧通入量在4 mg/L及以上时，TAN去除率显著低于其余组（P＜0.05）；臭氧剂量2 mg/L及以上时，TCBS平板上未检测到副溶血弧菌（从108 CFU/mL降低到不能被检测出的水平）。综合考虑臭氧对滤料细胞死亡率、氨氮处理效率以及副溶血弧菌的杀菌率， MBBR池臭氧推荐使用浓度为2 mg/L。 

关键词: MBBR, 臭氧, 硝化, 副溶血弧菌