Abstract: The phenomenon of oxygen deficiency is common in intensive aquaculture and live fish transportation, and has become an increasingly important issue in the aquaculture industry. The effects of hypoxic stress on water quality, biochemical parameters, and tissue structure during the transport of Siberian hybrid sturgeon (♀Acipenser baerii × ♂Acipenser schrenckii) larvae were investigated. The hybrid sturgeon larvae were placed in environments with dissolved oxygen concentrations of 2.5 ± 0.5 mg/L and 7.5 ± 0.5 mg/L for simulated transportation. Sampling was conducted before transport (0 h) and at 3, 6, 9, and 12 hours post-transport. The results showed that after 12 hours of transport, the survival rate of larvae in the hypoxic group was 54%. The serum cortisol (COR) concentration, liver alkaline phosphatase (AKP) and acid phosphatase (ACP) activities were significantly higher than those before transport (P<0.05). Serum glucose (GLU) levels peaked at 6 hours post-transport. Liver malondialdehyde (MDA), glutathione (GSH) content, and superoxide dismutase (SOD) activity were significantly higher than those in the normoxic control group (P<0.05). The liver antioxidant capacity (T-AOC) and lysozyme (LZM) activity in the hypoxic group exhibited a trend of initial decrease followed by an increase. After 12 hours of transport, the muscle glycogen content in the hypoxic group was significantly lower than that in the normoxic control group (P<0.05), while lactic acid levels peaked at 3 hours. More severe tissue damage was observed in the liver of hypoxic group larvae after 12 hours of transport. The study indicates that hypoxic stress during transportation affects juvenile fish survival rates, water quality, and other parameters, leading to oxidative stress responses and exacerbating liver tissue damage. 

Key words: juvenile hybrid sturgeon, low oxygen ;simulated transportation, oxidative stress, energy metabolism

摘要： 缺氧现象普遍存在集约养殖和活鱼运输中，已成为水产养殖业日益关注的问题。该研究探讨了低氧胁迫对西伯利亚杂交鲟(♀Acipenser baerii×♂Acipenser schrenckii )幼鱼不同运输时间下水质、生化参数、组织结构等的影响。将杂交鲟幼鱼置于溶氧质量浓度为2.5 ± 0.5 mg/L、7.5 ± 0.4 mg/L的环境下进行模拟运输。结果显示，运输12 h后，低氧组中幼鱼存活率为54 %，血清皮质醇( COR )浓度、肝脏碱性磷酸酶( AKP )和酸性磷酸酶( ACP )活性显著高于运输前( P<0.05 )；血清葡萄糖( GLU )含量在6 h后达到最大值; 肝脏丙二醛( MDA )浓度、微量谷胱甘肽( GSH )含量和超氧化物歧化酶( SOD )活性显著高于常氧组( P<0.05 )；低氧组肝脏抗氧化能力( T-AOC )、溶菌酶( LZM )活性均呈现先降低后升高的趋势；运输12 h后，肌肉糖原含量显著低于常氧组( P<0.05 )，乳酸含量在3 h时达到最高；低氧组幼鱼肝脏在运输12 h后出现更严重的组织损伤。研究表明运输过程中低氧胁迫会影响幼鱼的存活率、水质等参数，导致鱼体产生氧化应激反应，加剧肝脏组织损伤。

关键词: 杂交鲟幼鱼, 低氧, 模拟运输, 氧化应激, 能量代谢, 水质