摘要： 在开展圆斑星鲽(Verasper variegatus)无水活运技术研究过程中，为延长其保活时间，提高保活率，以达到长途运输充分实现鲜活圆斑星鲽经济价值的目的。本研究采用冷驯化、添加维生素C、麻醉剂及节律调控等方法对圆斑星鲽进行无水活运前处理，以保活时长及存活率为评价指标，筛选最优处理手段。结果显示：在冷驯化处理过程中，以1 ℃/h速率降温，无水活运24~36 h，存活率为83.33%，是未经冷驯化处理的两倍。以冷驯化为基础，辅助添加300 mg/L维生素C和100 mg/L麻醉剂MS-222，24 h存活率提高至100%。此外，活运时辅以500 Lux光照强度，无水活运48 h，存活率为50%，较全暗组提高200%。研究表明，采用冷驯化、添加维生素C、麻醉剂及节律调控等处理技术均能够有效降低圆斑星鲽在运输中的应激反应，减少鱼体损伤，延长保活时间，提高运输存活率，可满足圆斑星鲽商业化运输便捷、绿色、高效的要求。其中采用500 Lux光照强度处理方法运输圆斑星鲽存活率最佳。

关键词: 圆斑星鲽, 生态冰温, 降温速率, 维生素C, 麻醉剂, 生物节律

Abstract: The technology of waterless live transportation of aquatic products has the advantages of low carbon environmental protection, safety and high efficiency. it is more important, it conforms to the current development trend of e-commerce, meets the needs of consumers in various places for fresh aquatic products, and facilitates the implementation of the express delivery service of live aquatic products. In order to prolong the survival time and improve the survival rate of Verasper variegatus, the purpose of long-distance transportation to fully realize the economic value of fresh V. variegatus was achieved in the research process of waterless transportation technology of V. variegatus. In the long-term research on the technology of waterless preservation of aquatic products, the research group found that fasting and cold acclimation of fish before preservation and transportation can effectively prolong the survival time and increase the survival percentage of fish during transportation. At the same time, the preservation effect is affected by aquatic products, development degree, season, growth water area and low temperature tolerance. In this study, cold acclimation, vitamin C addition, anesthetics and biorhythm regulation were used to treat V. variegatus before waterless transportation. The optimal treatment method was screened with the survival time and survival rate as evaluation indexes. The experimental results showed that []in the process of cold acclimation, the survival rate was 83.33%, which was twice as high as that without cold acclimation, when the cooling rate was 1 ℃/h and the water was not transported for 24-36 hours. Based on cold acclimation, supplemented with 300 mg / L vitamin C and 100 mg / L anesthetic MS-222, the 24-hour survival rate increased to 100%. In addition, the survival rate was 50%, which was 200% higher than that of the total dark group, supplemented with 500 Lux light intensity and 48 hours of waterless transport. The experiment shows that cold acclimation, addition of vitamin C, anesthetics and biorhythm regulation can effectively reduce the stress response of V. variegatus during transportation, reduce fish damage, prolong the survival time, and improve the survival rate of transportation, which can meet the requirements of convenient, green and efficient commercial transportation of V. variegatus. Among them, the 500 Lux light intensity treatment method was used to transport the best survival rate of V. variegatus.

Key words: Verasper variegatus, ecological ice-temperature, cooling rate, vitamin c, anesthetic, biorhythm