Abstract: o study the influence of electric anesthesia technology on the anesthetic effect and blood biochemical indexes of Pearl gentian grouper(♀ Epinephelusfuscoguttatus ×♂ E.lanceolatus), the grouper after being temporarily raised for 24 hours was put into the electric shock box, and the fish was electrocuted with standard pulsed DC voltage. The non electrocuted group was used as the control group, and the anesthetic rate, dormancy time, and 72 h survival rate were calculated as the evaluation indexes of anesthetic effect. Three groups of voltages of 100V, 110V, and 120V were selected to analyze the physiological stress of fish by measuring 6 blood biochemical indexes of glutamic oxaloacetic transaminase, alanine transaminase, lactate dehydrogenase, glucose, lactate, and cortisol in blood within 48 hours  The results showed that when the power-on time was constant, the higher the voltage was, the higher the anesthesia rate was, the longer the dormancy time was, and the lower the survival rate was . The three groups （100 V, 110 V, 120 V) can completely anesthetize the Pearl gentian grouper, and the 72 h survival rate is 100%. The average dormancy time of the 100 V experimental group was 17.0 ± 3.84 min, and all biochemical indexes were better than those of other experimental groups; Compared with the control group, except that the value of aspartate aminotransferase increased significantly compared with the control group, the activity of alanine aminotransferase and lactate dehydrogenase were significantly lower than the control group, and the contents of glucose, lactate, and cortisol in serum were not significantly different from the control group. The experiment shows that electric shock with 100 V standard pulse DC for 3 s has no permanent damage to the fish body. It is a short time and less harmful anesthesia method for pearl gentian grouper.

Key words: ♀Epinephelusfuscoguttatus×, ♂E.lanceolatus；electronarcosis；behavior；blood biochemical；stress；transportation of live fish

摘要： 为研究电麻醉工艺对珍珠龙胆石斑鱼麻醉效应与血液生化指标的影响，将暂养24 h后的石斑鱼投入电击箱，采用脉冲直流电压对鱼体进行电击，以未电击组作为对照组，通过计算麻醉率、休眠时间和72 h存活率作为其麻醉效应的评价指标，优选100 V、110 V和120 V 3组电压，通过测定血液中48 h内的谷草转氨酶、谷丙转氨酶、乳酸脱氢酶、葡萄糖、乳酸和皮质醇6项血液生化指标，分析鱼体的生理应激情况。结果显示：通电时间一定时，电压越大，麻醉率越高，休眠时间越长，存活率越低；试验鱼在电刺激期与电紧张期停留时间越短；100 V、110 V、120 V电压均能对珍珠龙胆石斑鱼完全麻醉且72 h存活率为100%。100 V试验组平均休眠时间17.0±3.84 min，且各项生化指标均优于其他试验组；与对照组相比，除谷草转氨酶数值较对照组明显上升外，谷丙转氨酶活力、乳酸脱氢酶活力均显著性低于对照组，血清中葡萄糖、乳酸以及皮质醇含量与对照组差异不显著。研究表明，采用100 V标准脉冲直流电电击3 s对鱼体没有永久性损伤，是一种耗时短、伤害小的珍珠龙胆石斑鱼的麻醉方式，可以提升珍珠龙胆石斑鱼保活运输过程中的品质与效率。

关键词: 珍珠龙胆石斑鱼, 电麻醉, 行为特征, 生化指标, 应激, 活鱼运输