In order to calibrate the discrete element simulation parameters of fish pellet feed and analyze the influencing factors of repose angle of fish pellet feed, this paper measured the repose angle of fish pellet feed based on stacking experiment. Taking Tongwei 150 model pellet feed as the research object, the funnel method, cylinder lifting method and suction plate method were used to realize the formation of the repose angle, and MATLAB software was used to process the accumulation image to determine the boundary contour of pellet feed. Plackett-Burman test was used to screen out three significant influencing factors: the static friction coefficient between feed and feed, the restitution coefficient between feed and feed, and the rolling friction coefficient between feed and feed. Finally, Box-Behnken test was used to construct the regression model of repose angle and significant influencing parameters. The optimal simulation parameters were determined by Design Expert optimization as the recovery coefficient between feed and feed is 0.28, the static friction coefficient between feed and feed is 0.31, and the rolling friction coefficient between feed and feed is 0.14, among which the static friction coefficient between feed and feed and its quadratic term have the greatest influence on the repose angle of granular feed. The experimental results provide a reference for the discrete element parameter calibration of fish pellet feed and the design of feed delivery and throwing equipment.