In order to improve the processing and utilization rate of low-value fish species caught from marine capture, rationally use the byproducts from surimi and surimi products processing, make full use fish endogenous enzymes, and seek for the low-carbon, high efficiency deep-processing method for low-value fish, Collichthys lucidus was taken as the object to study the hydrolysis conditions of fish protein using endogenous proteases. Under the condition of natural pH, single factor experiment was adopted to investigate the effects of liquid to sample ratio, temperature and hydrolysis time on the soluble protein/total protein value, and the conditions were optimized as following: liquid to sample ratio being 3.4, temperature 53℃, and time 267 min. Under the optimal conditions, the soluble protein/total protein value was up to 18.35%, and compared with the predicted value 18.55% with regression equation, the relative error was 1.1%, illustrating that the model can rather precisely predict the soluble protein/total protein value. Under such conditions, the measured degree of hydrolysis was 15.84% and the protein of passing 0.45μm microfiltration mass ratio was 9.83%. The results show that the hydrolysis process could provide technical support for the production of fish sauce, bioactive peptides, amino acid and so on.