In order to find out the optimal feeding frequency for factory farming Epinephelus sp, this experiment investigated the effects of feeding frequency on the growth and plasma antioxidant indices of Epinephelus malabaricus [initial body weight of (238.99± 5.32) g]. The fish were divided into 3 groups: F1, F2 and F3, which were respectively fed 1, 2 and 3 times every day and fed to satiety each time. Samples were successively collected and analyzed after feeding 28 d, 56 d, and 84 d. The results showed that during the three periods of 0-28 day, 29-56 day and  57-84 day, as feeding frequency increasing, the weight gain rate and specific growth rate increased significantly (P<0.05). During 0-28 d and 29-56 d, the protein efficiency ratio (PER) of F2 was significantly higher than that of F1 and F3 (P<0.05), while during 57-84 d, the PER of F2 was significantly higher than that of F1 (P<0.05) but had no significant difference from that of F3 (P>0.05). Feed efficiency of F2 was significantly higher than that of F1 and F3 during 0-28 d (P<0.05), while during 29-56 d and 57-84 d, was higher than that of F1 (P<0.05) but had no significant difference with that of F3 (P>0.05). By the 28th d, plasma antioxidant indices were not significantly affected by feeding frequency (P>0.05). By the 56th d, T-AOC and CAT of plasma showed no significant difference among the groups (P>0.05), while T-SOD of F1 was significantly higher than that of F3 (P<0.05) but had no significant difference from F2 (P>0.05),  MDA of F3 was significantly higher than that of F1 (P<0.05) but that of F1 and F2 had no significant difference (P>0.05), and GSH-PX of F2 was significantly higher than that of F1 (P<0.05) but that of F3 and F1, F3 and F2 had no significant difference (P>0.05). By the 84th d, plasma T-AOC and MDA were not significantly affected by feeding frequency (P>0.05), while plasma T-SOD and CAT of F2 were significantly higher than those of F1 and F3 (P<0.05), and plasma GSH-PX of F2 significantly higher that of F1 (P<0.05) and had no significant difference (P>0.05) from F3. Results of the present study suggest that the optimal feeding frequency is 2 times/d in the culture of Epinephelus malabaricus with an initial body weight of about 239 g.