The aims of this study were to test the feasibility of slightly acidic electrolyzed water directly used for sterilization and purification of live Japanese scallop. The live Japanese scallop as test object were treated with different physicochemical properties of slightly acidic electrolyzed water (SAEW), and then artificially dipped with Vibrio parahaemolyticus. The disinfection effect of slightly acidic electrolyzed water, the survival rate of the scallop, the variation of V. parahaemolyticus and the microflora in different stages of water treatment were tested or determined by statistical method and biochemical experiments. The results showed that the survival rates after 1min group, 2min group and 4min group treated with slightly acidic electrolyzed water were all 82%, 8min group was 80%, the survival rates of the scallop in slightly acidic electrolyzed water groups were higher than that of the control (infected) group. The time of electrolyzed water treatment was significantly positive correlated with the total number of bacteria and coliforms (p＜0.05), and was no significantly related to the effective chlorine concentration of the electrolytic water used in the process of treatment (p＞0.05). And the quantity of V. parahaemolyticus in the live scallop fell from >1100MPN/g to 28MPN/g along with time extending of treatment of the electrolyzed water. In this experiment, the main bacteria in the early stage of the scallop were Pseudomonas and Vibrio. After the treatment, bacteria in the scallop tended to be complex, the dominant bacteria changed and the total number of bacteria decreased. The specific spoilage organism (SSO) after 24h was Pseudomonas in the treated scallop or not. In summary, SAEW has a potential application in the purification of live Japanese scallop.