In order to obtain the optimal extraction process of fish skin gelatin, the gelatinization treatment of anglerfish, squid, and horse noodle fish skin was carried out by the response method, and the amount of enzyme addition, enzymolysis time, enzymolysis temperature, as well as the time and amount of gelatin extraction were optimized. Lifting temperature. The results showed that the optimal extraction process for gelatin from anglerfish skin: enzyme addition 0.6%, enzymolysis time 2h, enzymolysis temperature 40℃, gel extraction time 1h, gel extraction temperature 75℃; The optimal extraction technology of squid skin gelatin: enzyme addition amount 0.6%, enzymolysis time 2h, enzymolysis temperature 40℃, gelatin extraction time 1h, gelatin extraction temperature 75℃; The optimal extraction technology of horse noodle fish skin gelatin: enzyme addition amount 0.6%, enzymolysis time 1h, enzymolysis temperature 30℃, gelatin extraction time 2h, gelatin extraction temperature 65℃. Compared with the traditional acid method, alkali method and other methods, the yield of gelatin was 11.23%. The yield of anglerfish skin gelatin optimized by the response surface process was increased to 24.598%, and the yield of squid skin gelatin increased from 6.82% to 20.807%. The extraction rate of horse noodle fish skin gelatin was 11.227%. Studies have shown that the response surface process optimization method in this study can effectively improve the yield of gelatin and provide a reference for the extraction technology of aquatic products gelatin.