Fishery Modernization >

2020 , Vol. 47 >Issue 3: 58

DOI: https://doi.org/10.3969/j.issn.1007-9580.2020.03.009

Preliminary study on denitrification of overlying water by pond sediments

  • FU Xianmao1 ,
  • LUO Guozhi1 ,
  • 2 ,
  • 3 ,
  • LIU Wenchang1 ,
  • TAN Hongxin1 ,
  • 2 ,
  • 3
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  • (1 Shanghai Ocean University, Research and Development Center of Aquaculture Engineering of Shanghai, Shanghai 201306, China
    2 Shanghai Ocean University, Key Laboratory of Freshwater Aquaculture Germplasm Resources, Ministry of Agriculture and Rural Affairs, Shanghai 201306, China
    3 Shanghai Ocean University, National Demonstration Center for Experimental Fisheries Science Education, Shanghai 201306, China)

Online published: 2020-09-16

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Abstract

This study takes pond sediments as the object to study its effect on denitrification of overlying water and microbial community. In the experiment, baking soda (NaHCO3) was used to regulate the alkalinity of the water. The initial alkalinity of the overlying water (calculated as CaCO3) was 143.3 ± 7.10 mg/L in group A, 275.2 ± 22.01 mg/L in group B, 385.1 ± 28.01 mg/L in group C and 466.5 ± 61.50 mg/L in group D. The results showed that the rate of denitrification of overlying water by sediments was 93.31% in Group A, 93.54% in Group B, 94.88% in Group C and 93.53% in Group D. There was no significant difference between the groups (P>0.05). Addition of alkalinity had no significant effect on the nitrogen removal effect of sediments (P>0.05). High-throughput results showed that Proteobacteria, Bacteroidetes, Chloroflexi and Acidobacteria were the main dominant bacteria at the phylum level. The dominant genuses Thiobacillus and Thioalkalispira were both denitrifying bacteria mainly based on sulfur autotrophic denitrification. Studies have shown that pond sediments have an obvious denitrifying effect on overlying water, and there is no significant difference in alkalinity regulation.

Key words： pond sediment; denitrification; microbial community; sulfur autotrophic denitrification; alkalinity

Cite this article

FU Xianmao1 , LUO Guozhi1 , 2 , 3 , LIU Wenchang1 , TAN Hongxin1 , 2 , 3 . Preliminary study on denitrification of overlying water by pond sediments[J]. Fishery Modernization, 2020 , 47(3) : 58 . DOI: 10.3969/j.issn.1007-9580.2020.03.009

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