Fishery Modernization >

2019 , Vol. 46 >Issue 2: 73 - 79

DOI: https://doi.org/10.3969/j.issn.1007-9580.2019.02.012

Study on optimization of the extraction of protamine from Takifugu Flavidus

  • LIU Shuji1 ,
  • CHEN Wuying2 ,
  • XU Min1 ,
  • LI Hui2 ,
  • SU Yongchang1 ,
  • ZHANG Bochao1 ,
  • QIAO Kun1 ,
  • SU Jie1 ,
  • LIU Zhiyu1
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  • (1 Fisheries Research Institute of Fujian, National Research and Development Sub-center of Marine Fish Processing Technology (Xiamen), Key Laboratory of Cultivation and High-value Utilization of Marin Organisms in Fujian Province, Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen 361013, Fujian, China;
    2 College of Biological Science and Technology, Minnan Normal University. Zhangzhou 363000, Fujian, China)
     

Online published: 2019-06-05

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Abstract

To study the preparation technology of protamine of Tetraodontidae, a single factor experiment was conducted to analyze the effect of NaCl solution pH, H2SO4 concentration, H2SO4 volume, extraction temperature, extraction time and ethanol volume on extraction rate of protamine, with testis of Takifugu Flavidus as raw material and extraction rate of protamine as index. Based on the above basis, the response surface methodology was used to optimize the extraction conditions of protamine. The results showed that after optimization with response surface methodology, the optimal extraction conditions for protamine of Tetraodontidae were NaCl solution pH 8.5, H2SO4 concentration 0.6 mol/L, H2SO4 volume 4 times, extraction temperature 45℃, extraction time 2.4 h and ethanol volume 4 times. Under these conditions, the extraction rate of protamine was up to 19.07%. The study showed that the proposal of optimizing the protamine of Tetraodontidae with the response surface methodology was reasonable and feasible, and it would provide a reference for the development and utilization of Tetraodontidae testis.

Key words： Takifugu Flavidus; protamine; response surface optimization

Cite this article

LIU Shuji1 , CHEN Wuying2 , XU Min1 , LI Hui2 , SU Yongchang1 , ZHANG Bochao1 , QIAO Kun1 , SU Jie1 , LIU Zhiyu1 . Study on optimization of the extraction of protamine from Takifugu Flavidus[J]. Fishery Modernization, 2019 , 46(2) : 73 -79 . DOI: 10.3969/j.issn.1007-9580.2019.02.012

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