Epinephelus fuscoguttatus♀×Epinephelus lanceolatus♂ is predominantly a commercial species cultured in seawater. An stable auditory brainstem response (ABR) detection system in Epinephelus fuscoguttatus♀×Epinephelus lanceolatus♂ was established and sine wave acoustic stimulus signals of 100, 200, 300, 500, 800, 1 000, 1 500, 2 000, 3 000, 5 000 and 10 000 Hz were applied to test the auditory thresholds of 20 Epinephelus fuscoguttatus♀×Epinephelus lanceolatus♂ with body length of 32-35cm. The results showed that the response to the brainstem potential detection system was auditory electrophysiological response, and the auditory frequency range of all Epinephelus fuscoguttatus♀×Epinephelus lanceolatus♂ was 100-5 000 Hz, where the best hearing was observed at 100-2 000 Hz, and the most sensitive sound frequency was 300 Hz with threshold of 75.8 dB. ABR audiogram of Epinephelus fuscoguttatus♀×Epinephelus lanceolatus♂ was created based on the average auditory thresholds obtained. The study showed that the auditory thresholds and the audiogram of Epinephelus fuscoguttatus♀×Epinephelus lanceolatus♂ obtained have an important significance in studying effects of noise on animal welfare of Epinephelus in different aquaculture mode, and they are not only the direct data for evaluating the effect of noise on Epinephelus , but also can provide indirect reference for optimization of aquaculture environment.