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Effects of pH acute stress on the behavior and immune enzyme activity of Babylonia areolata#br#

  

  1. （1Tianjin Agricultural College, College of Fisheries, Tianjin 300384, China；
    2  Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization by the Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China;
    3Sanya Tropical Fisheries Research Institute, Sanya 572018, China
    4Huizhou College, College of Life Sciences, Huizhou 516007, China）
  • Online:2022-12-20 Published:2023-02-01

pH急性胁迫对方斑东风螺行为及免疫酶活性的影响

  1. (1 天津农学院水产学院，天津 300384；
    2 中国水产科学研究院南海水产研究所，农业农村部南海渔业资源开发利用重点实验室，广东 广州 510300；
    3 三亚热带水产研究院，海南 三亚 572426；
    4 惠州学院生命科学学院，广东 惠州516007)
  • 通讯作者: 马振华（1981—），男，研究员，从事海水鱼类繁育与发育研究。E-mail:zhenhua.ma@hotmail.com
  • 作者简介:丁瑞霞（1999—），女，硕士研究生，研究方向：海水鱼类繁育与发育。E-mail:582750695@qq.com
  • 基金资助:
    三亚市院地科技合作项目（2018YD19，2019YD21）；财政部和农业农村部国家现代农业产业技术体系项目；农业农村部财政专项（NHYYSWZZZYKZX2020）；农业行业标准制定和修订项目（20191818）；中国水产科学研究院中央级公益性科研院所基本科研业务费专资金（2020TD55）；国家（省）重点科技项目三亚市配套资金（2019PT61）；揭阳市科技计划重点项目（SKJCX020）

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Abstract: To reveal the acute toxicity and physiological changes of Babylonia areolata in response to low pH and high pH stress, the pH =8.0 control group and pH =5.0, 6.0, 7.0, and 9.0 were used as the experimental group to investigate the activities of four immune enzymes glutathione peroxidase (GSH-PX), catalase (CAT), peroxidase (POD) and acid phosphatase (ACP) in the body of the Babylonia areolata. The results of the experiment show that pH stress has a significant effect on immune enzyme activity at different times (P＜0.05), while different pH stresses have no significant effect on immune enzyme activity (P＞0.05). After pH acute stress, 1) after 6h of low pH and high pH water culture, there is a decrease in vitality and slow climbing, and death begins to appear after 48h; 2) The activity of GSH-PX in each experimental group shows a trend of "inhibition-induction"; 3) with the continuous extension of time, the CAT activity of each pH treatment group shows a trend of "induction-inhibition-induction"; 4) the POD activity generally show a trend of "induction-inhibition";5)the ACP activity of the low
pH treatment group (pH= 5.0, 6.0) show a trend of "induction-inhibition". This study has practical significance for the cultivation of Babylonia areolata, and also enriches the basic data on the immune performance of other shellfish.

Key words: Babylonia areolata, immune enzymes, behavior, pH acute stress

摘要： 为揭示方斑东风螺(Babylonia areolata) 在低pH和高pH胁迫时的急性毒性和生理变化，以pH=8.0为对照组，pH=5.0、6.0、7.0和9.0为试验组，测定方斑东风螺体内4种免疫酶：谷胱甘肽过氧化物酶(GSH-PX)、过氧化氢酶(CAT)、过氧化物酶(POD)和酸性磷酸酶(ACP)活性。结果显示：不同时间pH胁迫对免疫酶活性有显著影响（P＜0.05），而不同pH胁迫对免疫酶的活力没有显著影响（P＞0.05）；方斑东风螺经过pH急性胁迫后，在低pH和高pH的水体养殖6 h后都出现了活力下降、爬壁缓慢，而在48 h后开始出现死亡；各试验组GSH-PX活性均呈现“抑制-诱导”的变化趋势；随着时间的不断延长，各pH处理组CAT活力均呈现“诱导-抑制-诱导”的变化趋势；4）POD活性总体表现出了“诱导-抑制”的趋势；低pH处理组（pH=5.0、6.0）ACP活性表现出“诱导-抑制”的趋势。该研究对于方斑东风螺的养殖具有参考意义，同时丰富贝类免疫系统方面的基础资料。

关键词: 方斑东风螺(Babylonia areolata), 免疫酶, 行为, pH急性胁迫