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LIU Bin1,2, LIU Xinfu1,2, ZHANG Yuefeng 3, LIU Huang4,Huang Bin1,2, GAO Yingying1,2,5(1 Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture,#br# Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, Shandong, China;#br# 2 Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology, Qingdao 266071, Shandong, China;#br# 3 Qingdao Starfish Instruments Co., Ltd, Qingdao 266000, Shandong, China;#br# 4 Fishery Machinery and Instrument Institute, Chinese Academy of Fishery Sciences, Shanghai 200092, China;#br# 5 College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 200090, China)
刘滨1,2，刘新富1,2，张跃峰3，刘晃4，黄滨1,2，高莹莹1,2,5（1农业部海洋渔业可持续发展重点实验室，中国水产科学研究院黄海水产研究所，山东 青岛 266071；
2青岛市鱼类种子工程与生物技术重点实验室，山东 青岛266071；
3青岛海星仪器有限公司，山东 青岛 266000；
4中国水产科学研究院渔业机械仪器研究所，上海200092；
5上海海洋大学，水产与生命学院，上海 200090）
Abstract:
Epinephelus fuscoguttatus♀×Epinephelus lanceolatus♂ is predominantly a commercial species cultured in seawater. An stable auditory brainstem response (ABR) detection system in Epinephelus fuscoguttatus♀×Epinephelus lanceolatus♂ was established and sine wave acoustic stimulus signals of 100, 200, 300, 500, 800, 1 000, 1 500, 2 000, 3 000, 5 000 and 10 000 Hz were applied to test the auditory thresholds of 20 Epinephelus fuscoguttatus♀×Epinephelus lanceolatus♂ with body length of 32-35cm. The results showed that the response to the brainstem potential detection system was auditory electrophysiological response, and the auditory frequency range of all Epinephelus fuscoguttatus♀×Epinephelus lanceolatus♂ was 100-5 000 Hz, where the best hearing was observed at 100-2 000 Hz, and the most sensitive sound frequency was 300 Hz with threshold of 75.8 dB. ABR audiogram of Epinephelus fuscoguttatus♀×Epinephelus lanceolatus♂ was created based on the average auditory thresholds obtained. The study showed that the auditory thresholds and the audiogram of Epinephelus fuscoguttatus♀×Epinephelus lanceolatus♂ obtained have an important significance in studying effects of noise on animal welfare of Epinephelus in different aquaculture mode, and they are not only the direct data for evaluating the effect of noise on Epinephelus , but also can provide indirect reference for optimization of aquaculture environment.
Key words:
Epinephelus fuscoguttatus♀×Epinephelus lanceolatus♂,
auditory threshold,
auditory brainstem response,
audiogram
摘要： 珍珠龙胆石斑鱼是重要的海水养殖经济鱼类，通过建立稳定的珍珠龙胆石斑鱼听性脑干反应（auditory brainstem response, ABR）检测系统，分别施加100、200、300、500、800、1 000、1 500、2 000、3 000、5 000和10 000 Hz的正弦波声音刺激信号，并应用该系统检测20条体长为32~35 cm的珍珠龙胆石斑鱼的听觉阈值。结果显示：该脑干电位检测系统获得的响应为听觉电生理反应，所有珍珠龙胆石斑鱼的听频范围为100~5 000 Hz，其中最适听力范围为100~2 000 Hz，最敏感声音频率为300 Hz，听觉阈值约为75.8 dB。根据得到的平均听力阈值, 构建了珍珠龙胆石斑鱼的听性脑干反应听力曲线。研究表明：研究获得的珍珠龙胆石斑鱼听觉阈值及曲线对于研究噪声对不同养殖模式下石斑鱼的动物福利具有重要意义，不仅是评估噪声对石斑鱼影响的直接试验数据，也能为养殖环境的优化提供间接参考。
