Abstract: In order to explore the best extraction conditions of Defatted Antarctic Krill Protein (DKP) and increase the added value of defatted Antarctic krill powder. Using defatted Antarctic krill powder as the raw material, and the protein was extracted by alkali solution and acid precipitation. Taking the protein yield as the index, the single factor test was carried out on the four influencing factors of extraction temperature, extraction time, solid-liquid ratio and NaOH concentration. The extraction process was analyzed and optimized by response surface methodology. The actual optimum extraction conditions of DKP were optimized by response surface methodology as follows: extraction temperature 69 ℃, extraction time 65 min, solid-liquid ratio 1:15, NaOH concentration 2.2%. Through validation test, the actual protein yield was 31.93% ± 0.35%, with a relative error of 1.19%±1.01% from the theoretical value of 32.32%.  The results show that the regression model is accurate and can be used to guide the production practice, which is beneficial to improve the added value of the defatted Antarctic krill powder and promote the sustainable development of the Antarctic krill industry.

Key words: defatted Antarctic krill powder, protein, alkali dissolution and acid precipitation method, response surface methodology, extraction optimization

摘要： 为探究脱脂南极磷虾蛋白质（Defatted Antarctic Krill Protein, DKP）最佳提取条件，提高脱脂南极磷虾粉附加值。以脱脂南极磷虾粉为原料，采用碱溶酸沉法提取其蛋白质，以蛋白质得率为指标，对提取温度、提取时间、料液比、NaOH百分比浓度4个影响因素进行单因素试验并采用响应面法对提取工艺进行分析与优化。结果显示：通过响应面法优化得到DKP实际最佳的提取条件为提取温度69℃、提取时间65 min、料液比1:15、NaOH百分比浓度2.2%，通过验证试验得出此提取条件下实际蛋白质得率为31.93%±0.35%，与理论值32.32%相对误差为1.19%±1.01%。研究表明，该回归模型预测准确，可以用于指导生产实践，有利于提高脱脂南极磷虾粉附加值，推动南极磷虾产业的可持续发展。

关键词: 脱脂南极磷虾粉, 蛋白质, 碱溶酸沉法, 响应面法, 提取优化