论文

棒叶蕨藻变种对重金属Cu2+、Pb2+和Cd2+胁迫的生理响应

  • 王荣霞1 ,
  • 黄 敏1 ,
  • 2 ,
  • 陈傅晓1 ,
  • 3 ,
  • 谭 围1 ,
  • 3
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  • (1海南省海洋与渔业科学院,海南 海口 570203;
    2海南省热带海水养殖技术重点实验室,海南省热带海水养殖工程技术研究中心,海南 海口 570203;
    3南海生物资源开发与利用协同创新中心,广东 广州510275)
王荣霞(1987—),女,工程师,硕士,研究方向:大型海藻养殖与渔业生态环境。E-mail:103490682@qq.com

网络出版日期: 2022-09-21

基金资助

海南省重点研发计划项目(ZDYF2017027);海南省自然科学基金项目(20164167)

The Physical Responses of Caulerpa sertularoides f. Longipes to Heavy Metal (Cu2+、Pb2+、Cd2+) Stress

  • WANG Rongxia1 ,
  • HUANG Min1 ,
  • 2 ,
  • CHEN Fuxiao1 ,
  • 3 ,
  • TAN Wei1 ,
  • 3
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  • (1. Academy of Marine and Fishery of Hainan Province, Haikou 570203, China;
    2. Hainan Provincial Key Laboratory for Tropical Seawater Aquaculture Technology, Hainan Provincial Research Center for tropical seawater aquaculture Engineering technology, Haikou 570203, China;
    3. South China Sea Bio-resource Exploitation and Utilization Collaborative Innovation Center, Guangzhou 510275, China)

Online published: 2022-09-21

摘要

为研究棒叶蕨藻变种(Caulerpa sertularoides f. Longipes)对重金属离子胁迫的生理响应,通过恒温光照培养方法探讨棒叶蕨藻变种在重金属铜离子(Cu2+)、铅离子(Pb2+)、镉离子(Cd2+)的胁迫下对其日特定生长率(SGR)、可溶性蛋白含量、叶绿素a(Chl-a)、谷胱甘肽转移酶(GST)和超氧化物歧化酶(SOD)等指标的影响。结果显示,3种重金属离子的胁迫会影响藻体的正常生长,SGR均出现负增长,可溶性蛋白和Chl-a含量随处理浓度的增大而呈下降趋势,其中Cu2+的影响尤为明显;重金属对藻体生长的抑制能力大小为Cu2+>Pb2+>Cd2+;3种重金属离子对SOD和GST影响均表现为先升后降趋势,除了Cd2+对SOD影响峰值出现在5.00 mg/L之外,其他处理组的峰值均出现在浓度为2.50mg/L时。研究结果可为棒叶蕨藻变种的重金属胁迫机理研究提供参考。

本文引用格式

王荣霞1 , 黄 敏1 , 2 , 陈傅晓1 , 3 , 谭 围1 , 3 . 棒叶蕨藻变种对重金属Cu2+、Pb2+和Cd2+胁迫的生理响应[J]. 渔业现代化, 2017 , 44(2) : 25 -29 . DOI: 10.3969/j.issn.1007-9580.2017.02.005

Abstract

In order to study the physiological responses of the algae Caulerpa sertularoides f. Longipes to heavy metal (Cu2+, Pb2+, Cd2+) stress, constant-temperature illuminating cultivation method has been adopted to explore the influence of the stress from Cu2+, Pb2+ and Cd2+ on the specific growth rate (SGR), content of soluble protein, chlorophyll-a (Chl-a), glutathione-S-transferase (GST) and superoxide dismutase (SOD) of the algae. The results show that stress of the three kinds of heavy metal ions affects the normal growth of the algae; SGR showed negative growth; soluble protein and Chl-a contents decreased when the content of heavy metal ions increased; the influence of Cu2+ was most obvious. The inhibition ability of heavy metals on the growth of algae was as follows: Cu2+>Pb2+>Cd2+. Both the SOD and GST contents, under the influence of the three heavy metal ions, increased first and then declined; the peak effect on the SOD concentration appeared when the concentration of Cd2+was 5mg/L, but in other treatment groups the peak effect appeared at the concentration of 2.50mg/L. This study will provide a scientific basis for the study on the mechanism of heavy metal stress to Caulerpa sertularoides f. Longipes.
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