渔业现代化

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棒叶蕨藻变种对重金属Cu2+、Pb2+和Cd2+胁迫的生理响应

  

  1. （1海南省海洋与渔业科学院，海南 海口 570203；
    2海南省热带海水养殖技术重点实验室，海南省热带海水养殖工程技术研究中心，海南 海口 570203；
    3南海生物资源开发与利用协同创新中心，广东 广州510275）
  • 出版日期:2017-04-20 发布日期:2022-09-21
  • 通讯作者: 谭围（1982—），男，高级工程师，硕士，研究方向：水产经济动物繁殖生物学。E-mail:tanwei2599@163.com -------------
  • 作者简介:王荣霞（1987—），女，工程师，硕士，研究方向：大型海藻养殖与渔业生态环境。E-mail:103490682@qq.com
  • 基金资助:
    海南省重点研发计划项目（ZDYF2017027）；海南省自然科学基金项目（20164167）

The Physical Responses of Caulerpa sertularoides f. Longipes to Heavy Metal (Cu2+、Pb2+、Cd2+) Stress

  1. (1. Academy of Marine and Fishery of Hainan Province, Haikou 570203, China;
    2. Hainan Provincial Key Laboratory for Tropical Seawater Aquaculture Technology, Hainan Provincial Research Center for tropical seawater aquaculture Engineering technology, Haikou 570203, China;
    3. South China Sea Bio-resource Exploitation and Utilization Collaborative Innovation Center, Guangzhou 510275, China)
  • Online:2017-04-20 Published:2022-09-21

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摘要： 为研究棒叶蕨藻变种（Caulerpa sertularoides f. Longipes）对重金属离子胁迫的生理响应，通过恒温光照培养方法探讨棒叶蕨藻变种在重金属铜离子(Cu2+)、铅离子(Pb2+)、镉离子(Cd2+)的胁迫下对其日特定生长率（SGR）、可溶性蛋白含量、叶绿素a（Chl-a）、谷胱甘肽转移酶（GST）和超氧化物歧化酶（SOD）等指标的影响。结果显示，3种重金属离子的胁迫会影响藻体的正常生长，SGR均出现负增长，可溶性蛋白和Chl-a含量随处理浓度的增大而呈下降趋势，其中Cu2+的影响尤为明显；重金属对藻体生长的抑制能力大小为Cu2+>Pb2+>Cd2+；3种重金属离子对SOD和GST影响均表现为先升后降趋势，除了Cd2+对SOD影响峰值出现在5.00 mg/L之外，其他处理组的峰值均出现在浓度为2.50mg/L时。研究结果可为棒叶蕨藻变种的重金属胁迫机理研究提供参考。

关键词: 棒叶蕨藻变种, 重金属, 胁迫, 生理响应

Abstract: In order to study the physiological responses of the algae Caulerpa sertularoides f. Longipes to heavy metal (Cu2+, Pb2+, Cd2+) stress, constant-temperature illuminating cultivation method has been adopted to explore the influence of the stress from Cu2+, Pb2+ and Cd2+ on the specific growth rate (SGR), content of soluble protein, chlorophyll-a (Chl-a), glutathione-S-transferase (GST) and superoxide dismutase (SOD) of the algae. The results show that stress of the three kinds of heavy metal ions affects the normal growth of the algae; SGR showed negative growth; soluble protein and Chl-a contents decreased when the content of heavy metal ions increased; the influence of Cu2+ was most obvious. The inhibition ability of heavy metals on the growth of algae was as follows: Cu2+>Pb2+>Cd2+. Both the SOD and GST contents, under the influence of the three heavy metal ions, increased first and then declined; the peak effect on the SOD concentration appeared when the concentration of Cd2+was 5mg/L, but in other treatment groups the peak effect appeared at the concentration of 2.50mg/L. This study will provide a scientific basis for the study on the mechanism of heavy metal stress to Caulerpa sertularoides f. Longipes.

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