Abstract: In order to improve the efficiency of outdoor large-scale culture of Isochrysis zhanjiangensis, comparation of cell density for I. zhanjiangensis large-scale outdoor cultivation was conducted in present study, under three modes that including pipeline photobioreactor, polyethylene bucket and cement tank. The density of inoculated algae was 10.0×104 cells/mL and the cultivation experiment was lasted for 16 d. And in pipeline photobioreactor mode for I. zhanjiangensis, the highest productivity and specific growth rate were 0.054 3 g/L/d and 0.276 7 µ/d respectively, with 3.70×106 cells/mL for cell density and 1.90 mg/L, 0.94 mg/L, 0.95 mg/L, for total chlorophyll contents, chlorophyll a and b respectively. The maximum biomass dry weight was observed to 0.727 0 g/L, while 159.125 5 mg/L for the maximum total protein content and 42.862 7 mg/L for the maximum soluble sugar content. The best production was gain under the pipeline photobioreactor mode, followed by the cement pond mode. The pipeline photobioreactor mode is more helpful to improve productivity and accumulation for biomass and cell chlorophyll, compared with polyethylene bucket and cement tank culture mode. Furthermore, pipeline photobioreactor mode is easier to operate with high space utilization. In conclusion, pipeline photobioreactor mode is more suitable for I. zhanjiangensis large-scale outdoor cultivation, and support the development of mechanized and intelligent technology for microalgae production in the future.

Key words: Isochrysis zhanjiangensis, large-scale production, photobioreactor, cement tank, polyethylene bucket, productivity, photosynthetic pigment

摘要： 为进一步提高湛江等鞭金藻室外规模化培养效率，对管道式光生物反应器、聚乙烯桶和水泥池3种微藻室外规模化培养模式下湛江等鞭金藻的藻密度进行了评估比较。试验将接种藻密度控制为10.0×104 cells/mL，培养周期为16 d。结果显示：管道式光生物反应器培养模式下，生产力相对最高为0.054 3 g/L/d、比生长速率为0.276 7 µ/d、最高藻细胞密度为3.70×106 cells/mL、叶绿素a含量0.94 mg/L、叶绿素b含量0.95 mg/L、总叶绿素含量1.90 mg/L、最大生物量干重为0.727 0 g/L、最高总蛋白含量为159.125 5 mg/L、最大可溶性糖含量为42.862 7 mg/L。管道式光生物反应器规模化培养效果最佳，其次为水泥池模式。研究表明，管道式光生物反应器有利于生产力提高及生物质和叶绿素积累，且操作方便，空间利用率高，更适于微藻户外规模化培养，本研究可为微藻培养模式的机械化、智能化生产工艺的发展提供参考。

关键词: 湛江等鞭金藻, 规模化生产, 光生物反应器, 水泥池, 聚乙烯桶, 生产力, 光合色素