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BAI Chan1, XIONG Guangquan1, HUANG Min2, QU Chang1, LIAO Tao1,ZHANG Jinmu1(1 Institute of Agro-Products Processing and Nuclear-Agricultural Technology, Hubei Academy of Agricultural Sciences/Hubei Engineering Research Center for Farm Products Irradiation, Wuhan 430064, Hubei, China;#br# 2 Irradiation Preservation Technology Key Laboratory of Sichuan Province, Sichuan Institute of Atomic Energy, Chengdu 610101, Sichuan, China)
白婵1,熊光权1,黄敏2,曲畅1,廖涛1,张金木1(1湖北省农业科学院农产品加工与核农技术研究所,湖北省农产品辐照工程技术研究中心,湖北 武汉 430064;
2四川省原子能研究院辐照保藏四川省重点实验室,四川 成都 610101)
Abstract:
In order to seek for the best conditions for waterless preservation of Ictalurus punctatus anesthetized by eugenol, the survival rate and changes on physiological-biochemical parameters of Ictalurus punctatus after preserved for 5h at different temperatures (0℃, 2℃, 4℃, 6℃ and 8℃) and 24h after recovery were studied. The result indicated that the optimum temperature for 5h waterless preservation of Ictalurus punctatus anesthetized by eugenol was 2℃-6℃, at which the survival rate 24h after recovery could be ≥ 80%; after preserved for 5h, GPT and GOT in blood were significantly higher than those before preservation (P<0.05) , and 24h after recovery, at 2℃, GPT and GOT could respectively recovered to 12.52 U/L and 16.13 U/L ; the content of CREA and BUN increased dramatically (P<0.05), especially at 6℃; total fish protein, crude fat, hardness and chewiness were on a downtrend (P<0.05). The study suggested that waterless preservation at 2 ℃had less effect on physiological-biochemical parameters of fish.
Key words:
Ictalurus punctatus,
eugenol,
temperature,
preservation,
biochemical parameters
摘要: 为寻求丁香酚麻醉斑点叉尾鮰(Ictalurus punctatus)无水保活的最佳条件,研究了不同无水保活温度(0℃、2℃、4℃、6℃和8℃)下斑点叉尾鮰保活5 h以及复苏24 h后的存活率及各生理生化指标的变化。结果显示,丁香酚麻醉斑点叉尾鮰无水保活5 h的最佳保活温度在2℃~6℃,复苏24 h后存活率≥80%;保活5 h后,血液中谷丙转氨酶(GPT)和谷草转氨酶(GOT)显著高于保活前(P<0.05),复苏24 h后, 2℃保活组GPT和GOT分别可恢复至12.52 U/L和16.13 U/L;肌酐和尿素氮含量显著升高(P<0.05),尤其在6℃下;鱼肉总蛋白、粗脂肪、硬度、咀嚼性呈下降趋势(P<0.05)。研究表明,在2℃下无水保活,对鱼肉的生理生化指标影响较小。