The technology of waterless live transportation of aquatic products has the advantages of low carbon environmental protection, safety and high efficiency. it is more important, it conforms to the current development trend of e-commerce, meets the needs of consumers in various places for fresh aquatic products, and facilitates the implementation of the express delivery service of live aquatic products. In order to prolong the survival time and improve the survival rate of Verasper variegatus, the purpose of long-distance transportation to fully realize the economic value of fresh V. variegatus was achieved in the research process of waterless transportation technology of V. variegatus. In the long-term research on the technology of waterless preservation of aquatic products, the research group found that fasting and cold acclimation of fish before preservation and transportation can effectively prolong the survival time and increase the survival percentage of fish during transportation. At the same time, the preservation effect is affected by aquatic products, development degree, season, growth water area and low temperature tolerance. In this study, cold acclimation, vitamin C addition, anesthetics and biorhythm regulation were used to treat V. variegatus before waterless transportation. The optimal treatment method was screened with the survival time and survival rate as evaluation indexes. The experimental results showed that []in the process of cold acclimation, the survival rate was 83.33%, which was twice as high as that without cold acclimation, when the cooling rate was 1 ℃/h and the water was not transported for 24-36 hours. Based on cold acclimation, supplemented with 300 mg / L vitamin C and 100 mg / L anesthetic MS-222, the 24-hour survival rate increased to 100%. In addition, the survival rate was 50%, which was 200% higher than that of the total dark group, supplemented with 500 Lux light intensity and 48 hours of waterless transport. The experiment shows that cold acclimation, addition of vitamin C, anesthetics and biorhythm regulation can effectively reduce the stress response of V. variegatus during transportation, reduce fish damage, prolong the survival time, and improve the survival rate of transportation, which can meet the requirements of convenient, green and efficient commercial transportation of V. variegatus. Among them, the 500 Lux light intensity treatment method was used to transport the best survival rate of V. variegatus.