In order to explore the culture effect of tilapia in circulating flow water tank of pond, study on culture in circulating flow water tank of pond was carried out in 2017. There were 19 950, 34 713 and 40 166 species of GIFT tilapia with specification of (3.5±0.11) g respectively placed in three tanks, and 700 species of bighead carp and 1 330 000 larvae of Macrobrachium rosenbergii placed in ponds outside the tanks. The water quality indicators and aquaculture production of the culture system were analyzed and determined in the study. Results showed that during the culture period, the temperature of water was between 25.4 ℃ and 32.6 ℃, DO was between 4.41 and 7.22 mg/L, pH was between 7.33 and 7.94, , the mass concentration of ammonia nitrogen and nitrite nitrogen was from 0.11 to 2.07 mg / L and 0.004 to 0.107 mg / L respectively, and the water quality met the growth needs of tilapia; the study lasted for 165 d, the output of the three tanks was 4 481 kg, 8 796 kg and 10 632 kg respectively, FCR was 1.19, 0.95 and 0.88 respectively, and the average yield was 72.5 kg/m2; 850 kg Macrobrachium rosenbergii and 1 300 kg bighead carp were got in ponds outside the tanks; the output value was 189 955.7 yuan per hectare and the profit was 40 454.7 yuan, and the economical input-output ratio was 1: 1.25. The study showed that culture of tilapia in circulating flow water tank in pond is superior to traditional culture mode in improving the quality of culture environment, increasing aquaculture production and increasing culture benefit.