Crassostrea arakensis is one of the main fouling organisms in the ocean, which has a significant impact on marine aquaculture facilities and marine engineering equipment. Short wavelength and high-energy ultraviolet radiation (UV-C, 200-280 nm) can damage the DNA and protein structure of shellfish larvae, affecting their survival and attachment, and can be used to prevent biological fouling. This study selected the larvae of Crassostrea arakensis as the research object. The single variable method was used to study the lethal response of larvae under different intensities and doses of UV-C, as well as the differences in attachment rates of larvae under different UV-C irradiation frequencies and distances. The research results showed that there were significant differences (P <0.05) between some treatment groups and the control group during the observation time period in the 10 W shortwave UV-C irradiation experiment. After 24 hours, there were no surviving larvae in all treatment groups in the experimental units, and the survival rate of the control group was (26.3±1.9)%; In the 20W UV-C irradiation test, there was a significant difference in survival rates between the treatment group and the control group at all observation time periods (P <0.05). After 24 hours, there were no surviving larvae in all treatment groups in the experimental units, and the survival rate of the control group was (22.3±1.3)%; when the irradiation frequency reached 5 minutes/h, the average adhesion rate decreased from (12.83±1.17)% to (3.31±0.69)%, significantly reducing the adhesion rate; There was no significant difference in the irradiation effect of the attachment rate between the distance of 15 and 20 cm, and both groups were able to effectively reduce attachment compared to the control group. This study can provide references for the design of equipment for UV-C prevention and control of biological fouling.