In order to deeply understand the stress response and plasticity of sea fish under ship-vibration condition, and supply the basic data and theoretical basis for breeding process design and mode construction of large breeding platform on the deep sea, changes in blood and biochemical indexes of Oplegnathus punctatus under different vibration frequency and time conditions are studied by using a mechanical vibrator to simulate the ship conditions. The results show that after vibration for 5min, there is no significant difference between 10Hz group, 50Hz group and the control group in all of the indexes, but for 30Hz group, serum glucose is significantly decreased, cortisol and epinephrine are significantly increased, and muscle glycogen and hepatic glycogen show no significant difference; after vibration for 60min,  serum glucose of all groups is significantly decreased, cortisol and epinephrine are significantly increased, and muscle glycogen and hepatic glycogen show no significant difference; for water quality change, after vibration for 60min, the dissolved oxygen concentration and ammonia concentration are slightly increased, and water temperature, salinity and pH show no significant change. According to the results, effects of low frequency vibration on fish mainly act on lateral line organ by causing changes in water flow, thus stimulating the brain of Oplegnathus punctatus and producing stress. Short time vibration would not have any great effect on Oplegnathus punctatus, however, long time vibration would cause stress response of Oplegnathus punctatus to some extent.