In order to study the performance change of the evaporator in the CO2 heat pump for aquatic product cooking equipment, the Simulink dynamic simulation model of the CO2 heat pump was established, and the CO2 heat pump test bench for the aquatic product cooking was built. The test method was a combination of simulation calculation and test verification. In this aquatic product cooking equipment, the evaporation temperature was controlled to vary from -5 °C to -15 °C to study the effects of different evaporation temperatures on frosting performance, heating capacity, heat pump coefficient and compression ratio. The results showed that the frosting rate gradually increased when the evaporation temperature changed from -5 ℃ to -15 ℃. In the simulation and experiment, the heating capacity of heat pump cooking equipment increased by 0.73 kW and 0.70 kW respectively, the heat pump coefficient decreased by 0.29 and 0.32 respectively, and the compression ratio increased by 0.52 and 0.50 respectively. Among them, the maximum error rate between simulation and experiment was 11.79 %, and the minimum error rate was 7.71 %. The simulation and experiment are in good agreement. Studies have shown that: on the premise of meeting the demand for aquatic product processing, in order to improve the efficiency of the processing system, the evaporation temperature should be lowered; the CO2 heat pump simulation platform established can better simulate the operation status of the aquatic product cooking equipment, and can provide some guidance for practical application of the CO2 heat pump to aquatic product cooking.