The deep-sea aquaculture platform is a typical engineering ship with self-propelled capability and long-term mooring in fixed sea areas. Due to the diversity of working conditions, the analysis of seakeeping performance of the aquaculture platform is more complicated than that of the common ships. Based on the actual design project of aquaculture platform, this paper studies the seakeeping performance of aquaculture platform under self-propelled and mooring working conditions according to the three-dimensional potential flow theory, and then combines with the simulation analysis of frequency and time domain to evaluate the aquaculture platform’s seakeeping performance with personnel safety, equipment safety and aquaculture safety as indicators. The results show that the ship’s seakeeping performance is good under the  sea states 1 to 5, which can effectively ensure the safety indicators. Under the sea state 6, the ship’s sway angle exceeds the limit, at which time it can be handled by operation management or installation of anti-rolling device. This paper preliminarily builds a systematic seakeeping performance analysis method and evaluation system of aquaculture platform, which can provide a reference for the seakeeping performance design and optimization of other aquaculture platform project.