Shipping and weighing are important in the process of aquatic products trading. The short distance transport weighing system of live fish was designed to analyze and explain the overall structure design and working principle. The system consists of electric lifting device, pneumatic loading and unloading device, automatic weighing and measuring device and control device. The control part is based on the programmable logic controller (PLC)to drive the electric hoist and the cylinder to lift, load and unload the live fish separately, and convey the fish to the transport vehicle on shore along the inclined chute by self-weight for transshipment to the destination. The software part, based on LabVIEW visualization platform, is designed with a transport system display interface that has the functions of system communication and data storage operation. The application effect of the system shows that the single conveying capacity of 300 kg for the short distance transport weighting system of live fish and the transport amount of 2 t/h have improved the transport efficiency of live fish, reduced a lot of manpower and the mechanical damage rate of live fish, and improved the mechanization level of fishery production.