In order to investigate the effects of different feeding frequencies and levels on the growth and immunity of juvenile Leiocassis longirostris, Leiocassis longirostris with average body mass of 1.15g was raised in a cement pool (2 m × 1 m × 1 m) at a frequency of 2 times / d and 3 times / d, respectively. The feeding levels at each feeding frequency were 2%, 4%, 6%, 8%, and 10% of the body mass respectively; 60 fishes were placed in each treatment group and raised for 42d. The results showed that there was a secondary correlation between the terminal body mass, specific growth rate and feeding level of the test fish at feeding frequencies of 2 times/d and 3 times/d. The two curves almost coincided, which indicated that the effects of feeding frequency and feeding level on terminal body mass and specific growth rate of the test fish were the same. The serum MDA content was lower at the feeding frequency of 2 times/d and the feeding level of 4% and 6%.The serum T-SOD content was higher at the feeding frequency of 2 times/d and the feeding level of 4% and 6%. The serum LYS content was significantly higher than that of the other groups at the feeding level of 6% and the feeding frequency of 2 times/d and 3 times/d (P<0.05). In combination with growth and immunity indicators, under the conditions of this experiment, the feeding mode with the feeding frequency of 2 times / d and feeding level of 6% was more conducive to the growth of juvenile Leiocassis longirostris.