为构建微藻混合共培养系统 ,探讨其含抗生素鳗鱼养殖尾水脱氮除磷和资源化利用 中的应用潜力 ,将卵囊藻(Oocystis sp. )和三 角褐指藻(Phaeodactylum tricornutum)混合培养 ,探究该系统在处理含抗养殖尾水中的有效性 。通过监测系统氨氮、硝酸盐氮、亚硝酸盐氮、总氮、总磷、化学需氧量的去除效果 ,评估其脱氮除磷能力;监测微藻生长 ,评估系统固碳能力;通过监测微藻叶绿素 a 含量及抗氧化酶活性 ,探究微藻对抗生素的应激响应 。结果显示 :混合藻系统中氨氮的去除率达 100% ,处理后硝酸盐氮、亚硝酸盐氮、总氮、总磷和化学需氧量的质量浓度分别为 13. 50 ± 1. 17、0. 20±0. 01、15. 09±1. 14、0. 71± 0. 03 和 31. 57± 2. 51 mg/L,去除率分别为 86. 03%、67. 33%、86. 13%、94. 87%和50. 60%;处理后环丙沙星质量浓度为 4. 52±0. 45 mg/L,去除率达 90. 95%。卵囊藻生长对数期时碳( 以 CO2 计)捕获率达峰值 ,为 0. 47 g/(L ·d) 。微藻通过提高过氧化氢酶和过氧化物酶活性增强抗氧化能力 。研究表明 ,混合藻培养系统具有良好脱氮除磷效果和环丙沙星去除效果 ,为含抗养鳗尾水的资源化利用提供了新视角 ,具有重要的实际应用意义。

This study aims to construct a mixed culture system of Oocystis and diation Phaeodactylum tricornutum,and evaluate its potential for removing nitrogen and phosphorus as well as treating antibiotic-containing aquaculture tailwater. The nitrogen and phosphorus removal capacity was evaluated by monitoring water quality parameters(NH -N,NO -N,NO -N,PO- -P ,and CDO) ,assessing carbon capture capacity by monitoring microalgae growth,and exploring the stress response of microalgae to antibiotics by monitoring the chlorophyll a content and antioxidant enzyme activity. Results showed that the mixed algae system achieved a 100% removal rate of ammonia nitrogen. The concentrations after treatment of nitrate nitrogen, nitrite nitrogen,total nitrogen,total phosphorus,and chemical oxygen demand were 13. 50± 1. 17 mg/L,0. 20± 0. 01 mg/L,15. 09 ± 1. 14 mg/L,0. 71 ± 0. 03 mg/L,and 31. 57 ± 2. 51 mg/L,respectively,with corresponding removal efficiencies of 86. 03% , 67. 33% ,86. 13% ,94. 87% ,and 50. 60%. The concentration of CIP was 4. 52±0. 45 mg/L,with a removal efficiency of 90. 95%.The carbon capture rate(CO2 -equivalent) of Oocystis reached its peak with 0. 47 g/(L ·d) during the logarithmic growth phase. Microalgae enhanced their antioxidant capacity by increasing the activities of CAT and POD. This study indicates that the mixed algae culture system has a positive effect on nitrogen and phosphorus removal,as well as ciprofloxacin removal. It provides new perspectives for the resource utilization of aquaculture tailwater and important practical application.