为了开发基于小龙虾的深加工产品,本试验以即食小龙虾为对象,研究两种杀菌方式对小龙虾氨基酸营养品质、质构特性、感官评价等理化性质的影响,并对其挥发性风味物质进行顶空固相微萃取气相色谱质谱(HS-SPME-GC-MS)联用分析。结果显示:巴氏杀菌处理的即食小龙虾氨基酸营养品质、质构特性及感官质量均优于高温高压杀菌组,更能迎合消费者的口味;巴氏杀菌和高温高压杀菌的即食小龙虾分别检测出41和44种挥发性风味物质;巴氏杀菌处理的即食小龙虾的醇类、醚类和酯类物质显著高于(P<0.05)高温高压杀菌组,这可能是两者风味差异的主要原因。总之,采用巴氏杀菌处理的即食小龙虾品质更好,营养丰富,肉质鲜嫩,香味浓郁。
To develop deep-processed products based on Procambarus clarkii, the effects of the two sterilization methods on the physicochemical properties of ready-to-eat Procambarus clarkii, such as amino acid nutritional quality, texture characteristics and sensory evaluation, were studied, and the volatile flavor compounds were analyzed using headspace-solid phase micro-extraction (HS-SPME) combined with gas chromatography-mass spectrometry (GC-MS). The results showed that the amino acid nutritional quality, texture characteristics and sensory evaluation of the ready-to-eat Procambarus clarkii treated with pasteurization were better than those of the high-temperature and high-pressure sterilization group, which could better cater to consumer's taste. 41 and 44 kinds of volatile flavor compounds were identified from the ready-to-eat Procambarus clarkii treated with pasteurization and the ready-to-eat Procambarus treated with high temperature-high pressure sterilization, respectively. The alcohols, ethers and esters in the ready-to-eat Procambarus clarkii treated with pasteurization were significantly higher (P < 0.05) than those of the ready-to-eat Procambarus treated with high temperature-high pressure sterilization, which might be the main reason for the difference in flavors between the ready-to-eat Procambarus treated with the two sterilization methods. In conclusion, it was found that the ready-to-eat Procambarus clarkii treated with pasteurization was better in quality, which had abundant nutrients, fresh and tender meat, and strong flavors.