为揭示方斑东风螺(Babylonia areolata) 在低pH和高pH胁迫时的急性毒性和生理变化，以pH=8.0为对照组，pH=5.0、6.0、7.0和9.0为试验组，测定方斑东风螺体内4种免疫酶：谷胱甘肽过氧化物酶(GSH-PX)、过氧化氢酶(CAT)、过氧化物酶(POD)和酸性磷酸酶(ACP)活性。结果显示：不同时间pH胁迫对免疫酶活性有显著影响（P＜0.05），而不同pH胁迫对免疫酶的活力没有显著影响（P＞0.05）；方斑东风螺经过pH急性胁迫后，在低pH和高pH的水体养殖6 h后都出现了活力下降、爬壁缓慢，而在48 h后开始出现死亡；各试验组GSH-PX活性均呈现“抑制-诱导”的变化趋势；随着时间的不断延长，各pH处理组CAT活力均呈现“诱导-抑制-诱导”的变化趋势；4）POD活性总体表现出了“诱导-抑制”的趋势；低pH处理组（pH=5.0、6.0）ACP活性表现出“诱导-抑制”的趋势。该研究对于方斑东风螺的养殖具有参考意义，同时丰富贝类免疫系统方面的基础资料。
丁瑞霞1
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2
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3
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黄星美2
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3
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赵旺2
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3
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邓正华2
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3
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陈恒大3
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温为庚2
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3
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马振华2
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3
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王江勇4
. pH急性胁迫对方斑东风螺行为及免疫酶活性的影响[J]. 渔业现代化, 2022
, 49(6)
: 84
-90
.
DOI: 10.3969/j.issn.1007-9580.2022.06.011
To reveal the acute toxicity and physiological changes of Babylonia areolata in response to low pH and high pH stress, the pH =8.0 control group and pH =5.0, 6.0, 7.0, and 9.0 were used as the experimental group to investigate the activities of four immune enzymes glutathione peroxidase (GSH-PX), catalase (CAT), peroxidase (POD) and acid phosphatase (ACP) in the body of the Babylonia areolata. The results of the experiment show that pH stress has a significant effect on immune enzyme activity at different times (P＜0.05), while different pH stresses have no significant effect on immune enzyme activity (P＞0.05). After pH acute stress, 1) after 6h of low pH and high pH water culture, there is a decrease in vitality and slow climbing, and death begins to appear after 48h; 2) The activity of GSH-PX in each experimental group shows a trend of "inhibition-induction"; 3) with the continuous extension of time, the CAT activity of each pH treatment group shows a trend of "induction-inhibition-induction"; 4) the POD activity generally show a trend of "induction-inhibition";5)the ACP activity of the low
pH treatment group (pH= 5.0, 6.0) show a trend of "induction-inhibition". This study has practical significance for the cultivation of Babylonia areolata, and also enriches the basic data on the immune performance of other shellfish.
